Monoclonal Antibody to Microcystin-LR (MC10E7)Product Specification
CLONE: MC10E7
ISOTYPE: Mouse IgG1
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with 200μl PBS containing sodium azide.
IMMUNOGEN: Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin.
SPECIFICITY: Recognizes all 4-Arg microcystins.
APPLICATION: ELISA (1:10'000-1:100'000)
Microcystin concentrations can be measured in the range of ~0.006μg/l to 0.3μg/l. The ELISA sensitivity is about 1'000 times higher compared to UV detection.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Storage at 4°C will decrease antibody activity and must be avoided.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Description With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC50 values for microcystin-LR of 0.06μg/l have been obtained. The provisional guideline value proposed by the WHO is 1 μg/l for drinking water. The detection limit for microcystin-LR is 0.006 μg/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7x1010 l/mol.
The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1μg/l were measured and a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.
Product Specific Literature References Highly sensitive immunoassay based on a monoclonal antibody specific for [4-arginine]microcystins: A. Zeck, et al.; Anal. Chim. Acta 441, 1 (2001)
Development of a direct competitive microcystin immunoassay of broad specificity: M.G. Weller, et al.; Anal. Sci. 17, 1445 (2001) Abstract
General Information
Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa. 最好的质量，最优的服务,更好地为广大新老客户提供服务如有更多其它试剂产品需求与查询,请及时接洽：info@express-cn.com 或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/lyb.htm ）留下您的查询内容，我们即会在当天为您作出回复。**********************************************************************************************************伊普瑞斯科技有限公司info@express-cn.com 在线客服：QQ：1091590259 1069267096 QQ：1650385705MSN：cindylee-1981@hotmail.com 联系人：孙先生总机：010-60206266 010-60206773www.express-cn.com
Microcystin微囊藻毒素-LR单克隆抗体