酪胺酸酶Tyrosinase from mushroom BioChemika, lyophilized, ≥2000 units/mgDescriptionsOther Notes Kinetic study of the suicide inactivation of tyrosinase1 ; Chromogenic assay
Unit Definition 1 U corresponds to the amount of enzyme which increases the absorbance at 305 nm by 0.001 per minute at pH 6.5 and 25°C (L-tyrosine as substrate, 3.0 mL reaction mix); 30 absorbance-U as described above are equivalent to ~1 U [when 1 U is the amount of enzyme which oxidyzes 1 μmol 4-methylcatechol per minute at pH 6.5 and 25°C]
General description Isoelectric point (pI): 4.7-5
pH optimum is 6-7Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.
Properties
mol wt
Mr ~125000
solubility
0.02 M phosphate, pH 6.5: 5mg/mL, clear, slightly brown to deep brown
storage temp.
?20°C
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酪胺酸酶Tyrosinase from mushroom