TOYOPEARL AF-Blue HC650M 亲和填料是一种非常有效的蛋白质纯化方法。其分离的机制是基于蛋白质和某些特定分子具有特异性的相互作用。除了生物特异性配体外（如酶底物、抗体、受体等等），一些俗称为类生物配体（如凝集素、染料）的物质也用在亲和层析中，而在固定化的金属离子上进行的类亲和层析在蛋白质和多肽的分离纯化工作中越来越重要。 亲和层析的选择性和效率极高。
一般来说, TOYOPEARL AF-Tresyl-650M和TOYOPEARL AF-Formyl-650M被推荐用来偶联蛋白质, 而TOYOPEARL AF-Epoxy-650M适合连结小分子配体, TOYOPEARL AF-Amino-650M和TOYOPEARL AF-Carboxyl-650M既可以偶联蛋白质也可以用来连接小分子配体。
TOYOPEARL亲和树脂所能偶联的代表性物质的偶联密度
结合了特异性配体的TOYOPEARL亲和树脂
TOYOPEARL AF-Chelate-650M树脂
对于TOYOPEARL AF-Chelate-650M来说，共价连接在介质上的亚氨基二乙酸（IDA）是金属离子的配体，可以结合各种金属离子(常用的为Ca2+, Ni2+, Zn2+, Co2+)，而金属离子的空轨道可以用来螯合特定的含有组氨酸和半胱氨酸的蛋白质和多肽。常用于纯化人重组生长因子, 组织蛋白酶原促进因子,血型糖蛋白和整体细胞.
TOYOPEARL AF Blue HC-650M树脂
功能团为Cibachron Blue F3GA. 结合蛋白容量高,尤其适合纯化白蛋白.
TOYOPEARL AF-Red 650M树脂
功能团为Procion Red HE-3B,适合纯化核苷依赖性酶, 血浆酶原脂蛋白,多肽, 荷尔蒙和细胞毒素
部分产品型号如下：
|
货号 |
生产商 |
描述 |
|
008000 |
TOSOH |
AF-Epoxy-650M 10G |
|
008002 |
TOSOH |
AF-Amino-650M 25mL |
|
008004 |
TOSOH |
AF-Formyl-650M 25mL |
|
008006 |
TOSOH |
AF-Carboxy-650M 25mL |
|
008038 |
TOSOH |
AF-Epoxy-650M 100G |
|
008039 |
TOSOH |
AF-Amino-650M 100mL |
|
008040 |
TOSOH |
AF-Formyl-650M 100mL |
|
008041 |
TOSOH |
AF-Carboxy-650M 100mL |
|
008651 |
TOSOH |
AF-Red-650ML 25mL |
|
014471 |
TOSOH |
AF-Tresyl-650M 5G |
|
014472 |
TOSOH |
AF-Tresyl-650M 100G |
|
014475 |
TOSOH |
AF-Chelate-650M 25mL |
|
014905 |
TOSOH |
AF-Tresyl-650M 200G |
|
014906 |
TOSOH |
AF-Tresyl-650M 1000G |
|
014907 |
TOSOH |
AF-Chelate-650M 1L |
|
014908 |
TOSOH |
AF-Chelate-650M 5L |
|
017396 |
TOSOH |
AF-Formyl-650M 1L |
|
017397 |
TOSOH |
AF-Formyl-650M 5L |
|
018074 |
TOSOH |
AF-Amino-650M 1L |
|
018315 |
TOSOH |
AF-Epoxy-650M 1000G |
|
018316 |
TOSOH |
AF-Amino-650M 5L |
|
018827 |
TOSOH |
AF-Carboxy-650M 1L |
|
018828 |
TOSOH |
AF-Carboxy-650M 5L |
|
019688 |
TOSOH |
AF-Blue HC650M 25mL |
|
019689 |
TOSOH |
AF-Blue HC650M 100mL |
|
019690 |
TOSOH |
AF-Blue HC650M 1L |
|
019691 |
TOSOH |
AF-Blue HC650M 5L |
|
019800 |
TOSOH |
AF-Chelate-650M 100mL |
|
019801 |
TOSOH |
AF-Red-650ML 100mL |
|
020030 |
TOSOH |
AF-Heparin HC-650M 10mL |
|
020031 |
TOSOH |
AF-Heparin HC-650M 100mL |
|
020032 |
TOSOH |
AF-Heparin HC-650M 1L |
|
020033 |
TOSOH |
AF-Heparin HC-650M 5L |
|
021384 |
TOSOH |
ToyoScreen AF-Chelate-650M, 1mL*6 |
|
021385 |
TOSOH |
ToyoScreen AF-Chelate-650M, 5mL*6 |
|
021386 |
TOSOH |
ToyoScreen AF-Blue HC-650M, 1mL*6 |
|
021387 |
TOSOH |
ToyoScreen AF-Blue HC-650M, 5mL*6 |
|
021388 |
TOSOH |
ToyoScreen AF-Red-650M, 1mL*6 |
|
021389 |
TOSOH |
ToyoScreen AF-Red-650M, 5mL*6 |
|
021390 |
TOSOH |
ToyoScreen AF-Heparin HC-650M, 1mL*6 |
|
021391 |
TOSOH |
ToyoScreen AF-Heparin HC-650M, 5mL*6 |
|
042102 |
TOSOH |
AF-Red-650ML 1L |
|
043400 |
TOSOH |
AFFIPAK ACT (AF-Epoxy, Tresyl-650M) , 65μm |
|
043402 |
TOSOH |
AF-Epoxy-650M 5G |
|
043410 |
TOSOH |
AFFIPAK (AF-Amino, Carboxyl, Formyl-650 M), 65μm |
|
043411 |
TOSOH |
AF-Amino-650M 10mL |
|
043412 |
TOSOH |
AF-Carboxy-650M 10mL |
|
043413 |
TOSOH |
AF-Formyl-650M 10mL |