相较于目前市场上的原子力显微镜成像设备,HS-AFM突破了 “扫描成像速慢”的限制,扫描速度最高可达 20 frame/s,并且有 4 种扫描台可供选择。样品无需特殊固定染色,不影响生物分子的活性,尤其适用于生物大分子互作动态观测。液体环境下直接检测,超快速动态成像,分辨率为纳米水平。探针小,适用于生物样品;悬臂探针共振频率高,弹簧系数小,避免了对生物样品等的损伤。悬臂探针可自动漂移校准,适用于长时间观测。采用动态PID控制,高速扫描时仍可获得清晰的图像。XY轴分辨率2nm;Z轴分辨率0.5nm。
超高速视频级原子力显微镜HS-AFM推出至今,全球已有80多位用户,发表 SCI 文章 200 余篇,包括Science, Nature, Cell 等顶级杂志。
HS-AFM超高速视频级原子力显微镜应用案例:
1.Video imaging of walking myosin V 实时观察myosin V蛋白的运动
N. Kodera et al. Nature 468, 72 (2010). Kanazawa University
2.Real-space and real-time dynamics of CRISPR-Cas9 实时显示CRISPR基因编辑
Mikihiro et al. Nature Communications, (2017). Kanazawa University
3. High-speed atomic force microscopy shows that annexin V stabilizes membranes on the second timescale
Miyagi A, et al. Nature Nanotechnology (2016)
4. IgGs are made for walking on bacterial and viral surfaces
J Preiner, et al. Nature Communications(2014)
5. Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells
Mikihiro Shibata, et al. Scientific Reports(2015)
6. High-speed atomic force microscopy shows dynamic molecular processes in photoactivated bacteriorhodopsin
Shibata M, et al. Nature Nanotechnology (2010)
7. Tuning crystallization pathways through sequence engineering of biomimetic polymers
Xiang Ma, et al. Nature Materials (2017)
8. Lipid-bilayer-assisted two-dimensional self-assembly of DNA origami nanostructures
Yuki Suzuki, et al. Nature Communications(2015)
HS-AFM超高速视频级原子力显微镜设备规格及配置参数:
基本参数:
SS-NEX 型可选配置: