DNA Extractor (R) SP kit (血清、血浆的DNA提取试剂盒)Grade: for Genetic Research
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Wako Pure Chemical Industries, Ltd. (用于存在于血清或血浆中DNA片断的提取)Cat. No. Pkg. Size
296-60501 50Tests Summary
This product is for research use only. Do not administer it to human.DNA extraction from serum and plasmaDNA Extractor SP Kit efficiently extracts DNA fragments contained in serum and plasma. Based on the Sodium Iodide (NaI) method1, this kit enables the whole procedures done in a single micro-centrifuge tube without using hazardous phenol/chloroform.
Recently, tumor-specific genes have been amplified and detected in the serum and plasma of patients with various diseases such as lung, breast and colon cancer. Many of these reference articles are currently being published2,3,4.
The kit is a powerful pretreatment reagent for the detection and analysis of target DNA because of its high quality and yield.
Features
High DNA yield (about 100%) from small amount of serum and plasma
The DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA.Safe operation
No phenol or chloroform required.Minimum contamination: The entire extraction of DNA can be done in a single centrifuge tubeComplete removal of lipid derived from bloodLess variability in extraction from sample to sampleKit Contents (50 reactions)Enzyme reaction solution 1 x 10 mLProtein digestion solution 1 x 250 uL (uL: micro-L)Sodium iodide solution 1 x 15 mLAlcohol solution 1 x 30 mLWashing Solution (A) 1 x 50 mLWashing Solution (B) 1 x 50 mLReferencesIshizawa, M., Kobayashi, Y., Miyamura, T. and Matsuura, S.: Nucleic Acids Res., 22, 1774 (1994).Sozzi, G., Musso, K., Ratcliffe, C., Goldstraw, P., Pierotti, M. A. and Pastorino, U.: Clin. Cancer Res., 5, 2689 (1999)Silva, J. M., Dominguez, G., Garcia, J. M., Gonzalez, R., Villanueva M. J., Navarro, F., Provencio, M., San, Martin, S., Espana, P. and Bonilla, F.: Cancer Res., 59, 3251 (1999)Shao, Z. M., Wu, J., Shen Z. Z. and Nguyen, M.: Clin. Cancer Res., 7, 2222 (2001) 如有更多其它试剂产品需求与查询,请及时接洽:info@express-cn.com 或在我公司查询主页客户留言处(http://www.express-cn.com/expressgb/lyb.htm)留下您的查询内容,我们即会在当天为您作出回复。伊普瑞斯科技有限公司
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