DIG Oligonucleotide Tailing Kit, 2nd generation:Formulation containing recombinant Terminal Transferase Kit for 25 tailing reactions of 100 pmol oligonucleotides
产品号
产品规格
厂商
价格
03353583910
(3353583)
1 kit (25 tailing reactions)
Roche
5335
ApplicationOligonucleotides, tailed with DIG-dUTP, are used for DNA or RNA hybridization in membrane blots or in situ hybridization. BenefitsVery sensitive hybridization probes, due to the incorporation of several DIG-nucleotides Fast hybridization kinetics, due to the small size of oligonucleotides Single-stranded probes, no renaturation during hybridization Sequence can be designed according to the experiment Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells Product DescriptionTailing of oligonucleotides at the 3'-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100). Contents1. Reaction Buffer, 5x, 100 µl 2. CoCl2 Solution, 100 µl, 25 mM 3. DIG-dUTP Solution, 25 µl, 1 mM 4. dATP Solution, 25 µl, 10 mM 5. Recombinant Terminal Transferase, 25 µl, [400 U/µl] 6. Control Oligonucleotide, unlabeled, 25 µl, [20 pmol/µl] 7. Oligonucleotide, DIG-dUTP/dATP tailed, 20 µl, [2.5 pmol/µl] 8. Control DNA, 20 µl, [0.25 mg/ml] 9. Glycogen Solution, 50 µl, [20 mg/ml] 10. DNA Dilution Buffer, 3 x 1 ml, [50 µg/ml] fish sperm DNA 11. poly(A) Solution, 1 ml, [10 mg/ml] PrincipleDIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s).
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©Copyright 2000-2024 现代资讯 All Rights Reserved
QQ:369830746